Work Experience:
Research Specialist II: Institute for Advanced Learning and Research (IALR) 2006-present
- AFLP markers for rust resistance in Daylily
- Genetic diversity analysis of Daylily varieties
- Genome analysis of Lupinus albus germplasm collection using AFLP markers
- Construction of Subalpine Fir cDNA Library to amplify full length Matrix metalloproteases (MMPs) gene
- Construction of Loblolly Pine cDNA Library to amplify full length MMP gene
- Cloning of TIPM gene to generate transgenic Arabidopsis to see the inhibition of MMP
- Construction of cDNA Libraries from various Arabidopsis tissues for developmental regulation of MMP gene expression analysis in wild type and knock out mutants
- Cloning of At5MMP knock out (KO) gene for homozygous/heterozygous mutants analysis in Arabidopsis
- Cloning of Zein gene in expression vector.
- Cloning of omega 3 fatty acid (3 FAD genes) in binary vector for transform into plants
Researcher I: Department of Microbiology, Southern Illinois University, Carbondale, Illinois, 2003 to 2005
- Characterization of the pflDC genes regulation in E. coli
- Gene fusion for characterization of unknown regulatory regions and proteins
- Transduction analysis in bacteria for resistance to antibiotic and mutant screening
- Bacterial culturing under aerobic and anaerobic conditions
Research Assistant and Manager Automated DNA Sequencing Facility: Plant Biotechnology and Genome Center, Southern Illinois University, Carbondale, Illinois 1999 to 2003
- Analysis of soybean laccase (diphenol oxidase), a candidate for sudden death syndrome resistance gene Rfs2
- DNA sequencing using ABI 377 automated DNA sequencers
- Marker assisted selection of soybean varieties using SSR markers
- Gene Expression analysis in soybean roots
- Gene expression analysis in rat livers
Research Assistant: Laboratory for Crop Genome Analysis, Crop Biotechnology Center, Texas A&M University, College Station, Texas. 8/1998 to 12/1998
- Sequencing DNA and EST libraries using automated DNA sequencers
Laboratory Technician: Laboratory for Crop Genome Analysis, Crop Biotechnology Center, Texas A&M University, College Station, Texas. 1/1998 to 7/1998
- General laboratory maintenance, media and reagents preparations
Publications:
Iqbal MJ, R Ahsan, AJ Afzal, A Jamai, K Meksem and DA Lightfoot (2009) Analysis of the activity of soybean laccase encoded within the Rfs2/rhg1 locus. Plant Genomics CIMB (in press)
Iqbal MJ, S Yaegashi, R Ahsan, K Shopinski, DA Lightfoot 2005 Root response to F. solani f. sp. glycines: Temporal accumulation of transcripts in partially resistant and susceptible soybean. Theoretical and Applied Genetics 110: 1429-1438.
Iqbal MJ, S Yaegashi, R Ahsan, DA Lightfoot and WJ Banz 2002 Differentially abundant mRNAs in rat liver in response to diets containing soy protein isolate. Physiological Genomics 11: 219-226.
Iqbal MJ, S Yaegashi, VN Njiti, R Ahsan, KL Cryder and DA Lightfoot 2002 Resistance locus pyramids alter transcript abundance in soybean roots inoculated by Fusarium solani f. sp. glycines. Molecular Genetics and Genomics 268: 407-417.
Iqbal, MJ, AJ Afzal, S Yaegashi, E Ruben, K Triwitayakorn, VN Njiti, R Ahsan, AJ Wood and DA Lightfoot 2002 A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains Myo-inositol-1-phosphate synthase expression in soybean roots. Theoretical and Applied Genetics 105: 1115-1123.
Published Genomic and EST Sequences:
Iqbal MJ, Afzal AJ, Ahsan R, Shultz J, Meksem K, Zobrist K and Lightfoot DA 2001 Large-insert soybean genomic library, Glycine max genomic survey sequence. BH771052-BH771134, BH614617 -BH614626, BH610092 -BH610129, BH860847- BH860901
Iqbal MJ, Yuan J, Afzal AJ, Ahsan R, Shultz J, Meksem K and Lightfoot, DA 2001 Fusarium solani f. sp. glycines BAC Library Fusarium solani f. sp. glycines, genomic survey sequence. BH214545 -BH214549
Iqbal MJ, Ahsan R, Lightfoot DA and Banz WJ 2001 Differentially expressed ESTs in rat liver in response to soy dietary supplements. BM414859- BM414916, BM423314- BM423319
Publications in proceedings and abstracts:
J Iqbal, K Shopinski, S Yaegashi, R Ahsan, M Shopinski, DA Lightfoot 2005 Root Response To Fusarium solani f. sp. glycines: Temporal Accumulation Of Transcripts In Partially Resistant And Susceptible Soybean Governs Seed Yield In Blends. Presented at "Plant & Animal Genome XIII Conference", San Diego, CA.
J Afzal, R Ahsan, S Kazi, C Langin, MJ Iqbal, DA Lightfoot 2005 Proteomic And Genomic Approaches To Molecular Breeding Of Resistance To Soybean Sudden Death Syndrome And Cyst Nematode In Elite Cultivars. Presented at "Plant & Animal Genome XIII Conference", San Diego, CA.
MJ Iqbal, S Yaegashi , KL Shopinski , R Ahsan , DA Lightfoot 2004 Soybean root's response to Fusarium solani f. sp. glycines inoculation: a comparison of gene expression in resistant and susceptible varieties. Presented at "Plant & Animal Genome XII Conference", San Diego, CA.
R Ahsan, MJ Iqbal , A Jamai, K Meksem, DA Lightfoot 2004 Cloning and expression of soybean laccase diphenol oxidase in yeast. Presented at "Plant & Animal Genome XII Conference", San Diego, CA.
MJ Iqbal, Gabe Martin, R. Ahsan, S. Yaegashi, David Lightfoot, William Banz 2003. Differentially abundant mRNAs in rat liver in response to diets containing soy protein isolate. Presented at "Plant & Animal Genome XI Conference", San Diego, CA.
MJ Iqbal, S. Yaegashi, R Ahsan, V Njiti, K Cryder, David Lightfoot 2003. Resistance loci pyramids alter transcript abundance in soybean roots inoculated by Fusarium solani f. sp. glycines. Presented at "Plant & Animal Genome XI Conference", San Diego, CA.
MJ Iqbal, AJ Afzal, R Ahsan, S Yaegashi, V Njiti, David Lightfoot 2003. A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains myo-inositol-1-phosphate synthase expression in soybean roots. Presented at "Plant & Animal Genome XI Conference", San Diego, CA.
MJ Iqbal, K Meksem, S Yaegashi, E Ruben, V Njiti, J Schultz, R Ahsan, DA Lightfoot 2001 Analysis of Differential Gene Expression in Soybean Roots in Response to Fusarium solani Infection by Micro-Arrays. Presented at "Plant & Animal Genome IX Conference", San Diego, CA.
MJ Iqbal, E Ruben, R Ahsan, VN Njiti, K Meksem, DA Lightfoot 2000 Identification of Putative Genes Confering Resistance to SDS of Soybean by Differential Display of mRNA. Presented at "Plant & Animal Genome VIII Conference", San Diego, CA.
